The protocol is optimised for fresh frozen tissue sections only.
Currently each array is made up by 1007 spots.
Each spot is a circle with a diameter of 100 µm and individual spots have a center to center distance of 200 µm.
In theory the current Spatial Transcriptomics protocol works for all tissue types that express poly-A positive mRNA. However, new tissue types require optimisation of the protocol due to their varying morphology. For this the Tissue Optimisation protocol has been developed, allowing cost-efficient optimisation before proceeding to a ST Library Preparation and sequencing.